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tsa plus fluorescein kit  (Akoya Biosciences)


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    Structured Review

    Akoya Biosciences tsa plus fluorescein kit
    Tsa Plus Fluorescein Kit, supplied by Akoya Biosciences, used in various techniques. Bioz Stars score: 96/100, based on 281 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/tsa/TSA+Plus+Fluorescein/pmc13683886-240-41-48
    Average 96 stars, based on 281 article reviews
    tsa plus fluorescein kit - by Bioz Stars, 2026-11
    96/100 stars

    Images

    Related Articles

    Amplification:

    Article Title: Submedius Thalamus Modulates Orbitofrontal Cortex Representations During Maternal Behavior in Mice
    Article Snippet: For ISH combined with anti-GFP staining, after hybridization and washing, sections were incubated overnight with horseradish peroxidase-conjugated anti-DIG (Roche Applied Science cat#11207733910, 1:500) and anti-GFP (Aves Labs cat#GFP-1010, 1:500) antibodies. .. Signals were amplified using TSA-plus Cyanine 3 (AKOYA Bioscience, NEL744001KT, 1:70 in 1× plus amplification diluent) for 25 min, followed by washing, and GFP-positive cells were visualized with anti-chicken Alexa Fluor 488 (Jackson Immuno Research cat#703-545-155, 1:250). ..

    Article Title: Submedius thalamic inputs shape the presynaptic architecture of layer 5 orbitofrontal cortex in mice
    Article Snippet: For ISH combined with anti-GFP staining, after hybridization and washing, sections were incubated overnight with horseradish peroxidase-conjugated anti-DIG (Roche Applied Science cat#11207733910, 1:500) and anti-GFP (Aves Labs cat#GFP-1010, 1:500) antibodies. .. Signals were amplified using TSA-plus Cyanine 3 (AKOYA Bioscience, NEL744001KT, 1:70 in 1× plus amplification diluent) for 25 min, followed by washing, and GFP-positive cells were visualized with anti-chicken Alexa Fluor 488 (Jackson Immuno Research cat#703-545-155, 1:250). ..

    Article Title: Orbitofrontal cortex influences dopamine dynamics associated with alloparental behavioral acquisition in female mice
    Article Snippet: For ISH combined with anti-GFP staining, after hybridization and washing, sections were incubated with horseradish peroxidase–conjugated anti-DIG (Roche Applied Science, catalog no. 11207733910, 1:500) and anti-GFP (Aves Labs, catalog no. GFP-1020, 1:500) antibodies overnight. .. Signals were amplified by TSA-plus Cyanine 3 (AKOYA Bioscience, NEL744001KT, 1:70 in 1× plus amplification diluent) for 25 min, followed by washing, and then GFP-positive cells were visualized by anti-chicken Alexa Fluor 488 (Jackson ImmunoResearch, catalog no. 703-545-155, 1:250). .. DNA templates (500 to 1000 ng) amplified by PCR were subjected to in vitro transcription with DIG (catalog no. 11277073910)–RNA labeling mix and T3 RNA polymerase (catalog no. 11031163001) according to the manufacturer’s instructions (Roche Applied Science).

    In Situ Hybridization:

    Article Title: Parallel labeled-line organization of sympathetic outflow for selective organ regulation in mice.
    Article Snippet: PBS containing 50 ng/ml of DAPI (#8417, Sigma-Aldrich) was used for counternuclear staining. .. For dual-color ISH, HRP-conjugated anti-flu antibody (at a dilution of 1:250; #NEF710001EA, AKOYA Bioscience) was used to detect Flulabeled RNA probes by a 1:70 TSA-plus biotin (#NEL749A001KT; AKOYA Bioscience), which was then visualized with streptavidinAF488 (at a dilution of 1:250; Life Technologies). .. After inactivation of HRP with a 2% sodium azide solution in PBS for 15min at 25 °C, HRPconjugated anti-Dig (at a dilution of 1:500; #11207733910, Roche Applied Science) and a 1:70 TSA-plus Cyanine 3 were used to detect Dig-labeled cRNA probes.

    Incubation:

    Article Title: Furan fatty acids reprogram hepatic lipid metabolism via acyl-CoA conjugates in obesity.
    Article Snippet: After 30 min blocking, the slides were incubated with anti- DIG- POD (Roche) at 1:400 dilution for 60 min at RT according to the manufacturer’s instructions. .. TSA- plus Cyanine 3 (Akoya Bioscience) substrate was added to the sections and incubated twice for 5 min each to amplify the fluorescence signal. ..


    Fluorescence:

    Article Title: Furan fatty acids reprogram hepatic lipid metabolism via acyl-CoA conjugates in obesity.
    Article Snippet: After 30 min blocking, the slides were incubated with anti- DIG- POD (Roche) at 1:400 dilution for 60 min at RT according to the manufacturer’s instructions. .. TSA- plus Cyanine 3 (Akoya Bioscience) substrate was added to the sections and incubated twice for 5 min each to amplify the fluorescence signal. ..



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    Image Search Results


    Journal: iScience

    Article Title: A multimodal in situ framework for spatial characterization applied on SARS-CoV-2 live-attenuated vaccines in the nasal mucosa

    doi: 10.1016/j.isci.2026.117730

    Figure Lengend Snippet:

    Article Snippet: Signal development of the HRP-conjugated antibody was achieved by stepwise incubation with Opal TSA-DIG Reagent (Akoya Biosciences) diluted 1:250 in RNAscope Multiplex TSA Buffer for 10 minutes at RT, RNAscope Multiplex FL v2 HRP Blocker for 25 min at 40 °C in the HybEZ oven, and Opal 780 Reagent (Akoya Biosciences) diluted 1:62,5 in 1x Antibody Diluent/Block (Akoya Biosciences) for 10 min at RT.

    Techniques: Plasmid Preparation, Virus, Indirect ELISA, Recombinant, RNAscope, Multiplex Assay, Software, Negative Control, Confocal, Laser-Scanning Microscopy